East I J, Fitzgerald C J, Berrie D A
CSIRO, Division of Tropical Animal Production, Long Pocket Laboratories, Indooroopilly, Qld., Australia.
Vet Parasitol. 1993 Sep;49(2-4):191-200. doi: 10.1016/0304-4017(93)90118-7.
The components of a soluble extract of adult Oesophagostomum radiatum were separated into four fractions by gel filtration chromatography on a Superose-12 column. The second fraction had a molecular weight range of approximately 60,000-65,000 as judged by SDS gel electrophoresis. Vaccination of calves with this fraction significantly (P < 0.05) reduced faecal egg output after challenge infection. In addition, the packed cell volume of vaccinated calves was higher than that of control calves (P < 0.05). The effect of three adjuvants on the vaccine activity was compared. Fraction 2 presented in dextran sulfate reduced the faecal egg count by 93.6% (P < 0.05), Fraction 2 in Freund's complete adjuvant reduced the faecal egg count by 27% (P = N.S.). Quil A was ineffective as an adjuvant.
成年辐射食道口线虫可溶性提取物的成分通过在Superose - 12柱上进行凝胶过滤色谱法分离成四个部分。通过SDS凝胶电泳判断，第二部分的分子量范围约为60,000 - 65,000。用该部分对犊牛进行疫苗接种后，在攻击感染后显著（P < 0.05）降低了粪便虫卵排出量。此外，接种疫苗的犊牛的红细胞压积高于对照犊牛（P < 0.05）。比较了三种佐剂对疫苗活性的影响。硫酸葡聚糖中的第二部分使粪便虫卵计数降低了93.6%（P < 0.05），弗氏完全佐剂中的第二部分使粪便虫卵计数降低了27%（P = 无显著性差异）。Quil A作为佐剂无效。
