Botha C J, Naude T W, Swan G E, Dauth J, Dreyer M J, Williams M C
Department of Pharmacology and Toxicology, Faculty of Veterinary Science, University of Pretoria, Republic of South Africa.
Vet Hum Toxicol. 1993 Oct;35(5):409-13.
The cupruretic effect of 2 copper chelators, d-penicillamine and trientine, was assessed in 12 fistulated South African Mutton Merino rams of approximately 6 mo age following copper loading. Each animal received 20 mg CuSO4.5H(2)0/kg body mass as an 0.5% m/v aqueous solution, intraruminally, daily for 35 d. The animals were randomly assigned to either a d-penicillamine (n = 4) or trientine treatment group (n = 4) or an unmedicated control group (n = 4). A separate group of 3 rams were kept as non-copper-loaded controls. Urinary copper excretion was measured before and during treatment. All the sheep were housed individually or placed intermittently on steel metabolic crates to facilitate urine collection. At the end of the trial the animals were euthanatized and specimens of organs collected for determination of copper concentrations. d-Penicillamine significantly (p < 0.05) increased urinary copper excretion. Trientine failed to increase copper excretion in the urine when compared to the unmedicated and non-copper-loaded control groups.
在给12只约6月龄的南非肉用美利奴瘘管公羊进行铜负荷后，评估了两种铜螯合剂——d-青霉胺和曲恩汀的促铜排泄作用。每只动物每天经瘤胃给予20 mg CuSO4·5H₂O/kg体重，以0.5% m/v的水溶液形式，持续35天。将这些动物随机分为d-青霉胺治疗组（n = 4）、曲恩汀治疗组（n = 4）或未用药对照组（n = 4）。另外一组3只公羊作为未进行铜负荷的对照。在治疗前和治疗期间测量尿铜排泄量。所有绵羊单独饲养或间歇性地置于钢制代谢笼中，以利于尿液收集。试验结束时，对动物实施安乐死，并采集器官标本以测定铜浓度。d-青霉胺显著（p < 0.05）增加了尿铜排泄量。与未用药和未进行铜负荷的对照组相比，曲恩汀未能增加尿铜排泄量。
