Song L N, Zhang J S, Lu J
Department of Pathophysiology, Second Military Medical University, Shanghai, China.
Zhongguo Yao Li Xue Bao. 1993 Jul;14(4):337-9.
The effects of steroid hormone calcitriol (Cal) and its analogue calcipotriol on human osteosarcoma cell line HOS-8603 were determined. When cells grew in monolayer culture in the presence of hormones, their proliferations were inhibited both in dose- and time-dependent manners. The cells showed marked morphologic changes after a 4-d treatment to apparently less transformed fibroblast-like ones. Anchorage-independent growth studies indicated that both Cal and calcipotriol at 10 nmol.L-1 inhibited colony formation by HOS-8603 cells. As a marker enzyme of the osteoblastic phenotype, alkaline phosphatase activity was induced in response to Cal or calcipotriol 100 nmol.L-1. These results suggested that Cal and calcipotriol play an important role in regulating growth and differentiation of HOS-8603 cells.
测定了类固醇激素骨化三醇（Cal）及其类似物卡泊三醇对人骨肉瘤细胞系HOS-8603的影响。当细胞在激素存在下进行单层培养时，其增殖受到剂量和时间依赖性的抑制。经过4天的处理后，细胞表现出明显的形态变化，明显转变为较少转化的成纤维细胞样细胞。非贴壁生长研究表明，10 nmol.L-1的Cal和卡泊三醇均抑制HOS-8603细胞的集落形成。作为成骨细胞表型的标志酶，碱性磷酸酶活性在100 nmol.L-1的Cal或卡泊三醇作用下被诱导。这些结果表明，Cal和卡泊三醇在调节HOS-8603细胞的生长和分化中起重要作用。
