Saari J T, Johnson W T, Reeves P G, Johnson L K
United States Department of Agriculture, Agricultural Research Service, Grand Forks Human Nutrition Research Center, ND 58202-9034.
Am J Clin Nutr. 1993 Dec;58(6):891-6. doi: 10.1093/ajcn/58.6.891.
To test the effect of food restriction on responses to dietary copper deficiency, male weanling rats were fed two amounts of dietary copper via five dietary-intake regimens ranging from ad libitum to 70% of ad libitum intake. Copper-deficient rats exhibited characteristic signs, including depressed organ copper content, reduced activity of copper-dependent enzymes, enlarged hearts, and anemia. Food restriction attenuated the cardiac enlargement, red blood cell defects, and reduction of superoxide dismutase activity in copper-deficient rats. Mineral and enzyme assays suggested that possible mechanisms for this amelioration are the correction of copper status and/or the improvement of antioxidant status. Also, food restriction depressed serum cholesterol and enhanced cytochrome c oxidase activity in both copper-adequate and copper-deficient rats, which compensated for effects of copper deficiency. A second experiment illustrated that the mortality associated with severe copper deficiency was also inhibited by food restriction.
为了测试食物限制对膳食铜缺乏反应的影响，对雄性断乳大鼠通过五种膳食摄入方案给予两种量的膳食铜，摄入量范围从自由摄食到自由摄入量的70%。缺铜大鼠表现出特征性症状，包括器官铜含量降低、铜依赖性酶活性降低、心脏增大和贫血。食物限制减轻了缺铜大鼠的心脏增大、红细胞缺陷和超氧化物歧化酶活性降低。矿物质和酶分析表明，这种改善的可能机制是铜状态的纠正和/或抗氧化状态的改善。此外，食物限制降低了血清胆固醇，并增强了铜充足和缺铜大鼠的细胞色素c氧化酶活性，这弥补了铜缺乏的影响。第二项实验表明，与严重铜缺乏相关的死亡率也受到食物限制的抑制。
