Hesse R A, Couture L P, Ellsworth S R, Duhamel G E, Lu W, Dickinson E O, Benfield D A
Schering Plough Animal Health, Elkhorn Research Center, Omaha, NE 68022.
Am J Vet Res. 1993 Oct;54(10):1623-9.
The ability of viral glycoproteins (VP) VP4/VP7 reassortant swine rotaviruses (RV) to induce cross-neutralizing antibody against parental serotypes was investigated in guinea pigs. Using selective culture conditions, we produced 10 reassortant viruses that contained gene segment 4 of the OSU RV strain and gene segment 9 of the Gottfried RV strain. These reassortant RV grew to high titer in cell culture and were neutralized by monospecific antisera against both parental RV strains. The reassortant RV were chemically inactivated with binary ethylenimine, adjuvanted with aluminum hydroxide, and used to produce antisera in guinea pigs. The hyperimmune antisera had high neutralization titer against both parent RV strains. These results indicate that several of the reassortant RV may be capable of inducing neutralizing antibodies to VP4 and VP7 and may have future use as bivalent vaccine strains.
在豚鼠中研究了病毒糖蛋白（VP）VP4/VP7重配猪轮状病毒（RV）诱导针对亲本血清型的交叉中和抗体的能力。利用选择性培养条件，我们产生了10种重配病毒，它们含有OSU RV株的基因片段4和Gottfried RV株的基因片段9。这些重配RV在细胞培养中生长至高效价，并被针对两种亲本RV株的单特异性抗血清中和。重配RV用双（2-氯乙基）胺化学灭活，用氢氧化铝佐剂，并用于在豚鼠中产生抗血清。高免疫抗血清对两种亲本RV株均具有高中和效价。这些结果表明，几种重配RV可能能够诱导针对VP4和VP7的中和抗体，并可能在未来用作二价疫苗株。
