Nishida Y, Kondo S, Miura Y, Iwata H, Isobe K
Department of Orthopaedic Surgery, Nagoya University School of Medicine, Japan.
Biochem Biophys Res Commun. 1993 Nov 15;196(3):1081-5. doi: 10.1006/bbrc.1993.2361.
The viability of syngeneic, allogeneic and xenogeneic bone grafts was investigated in mice and rats by the polymerase chain reaction method using carcinoembryonic antigen transgenic mice as donors. DNA of syngeneic grafts was detected more than 24 weeks after bone grafting. In contrast, the DNA of allogeneic thymic grafts and xenogeneic bone grafts disappeared by 3 weeks after transplantation. However, DNA of allogeneic bone grafts was detected until 20 weeks after transplantation. Histological examination at 12 weeks after surgery detected some donor nuclei in both syngeneic and allogeneic bone grafts.
通过聚合酶链反应方法，以癌胚抗原转基因小鼠作为供体，在小鼠和大鼠中研究了同基因、异基因和异种骨移植的生存能力。同基因移植骨的DNA在骨移植后24周以上仍可检测到。相比之下，异基因胸腺移植和异种骨移植的DNA在移植后3周消失。然而，异基因骨移植的DNA在移植后20周仍可检测到。术后12周的组织学检查在同基因和异基因骨移植中均检测到一些供体细胞核。
