H2B组蛋白基因的肝脏特异性反式作用因子在肝脏再生过程中重新合成。

Regenerating liver-specific transacting factors of H2B histone gene are newly synthesized during liver regeneration.

作者信息

Lim K, Park H C, Kim K Y, Lee M S, Kweon G R, Kwak S T, Hwang B D

机构信息

Department of Biochemistry, School of Medicine, Chungnam National University, Daejeon, Korea.

出版信息

Biochem Biophys Res Commun. 1993 Nov 15;196(3):1093-100. doi: 10.1006/bbrc.1993.2363.

DOI:10.1006/bbrc.1993.2363

PMID:8250866

Abstract

We have investigated DNA synthesis and levels of H2B histone mRNA, and the binding pattern of nuclear proteins to various elements in the rat H2B histone gene upstream region with DNase I footprinting assay. Both DNA synthesis and H2B histone mRNA level were increased with maximal stimulation reached at 24 hrs and 36 hrs after partial hepatectomy, respectively. In DNase I footprinting analysis, the nuclear factors interacting with the three elements, TATA at -19 bp (site AR), site B at -29 bp, and CAAT at -69 bp (site C) were required during maximal increase of H2B histone mRNA level after partial hepatectomy. The DNase I protection pattern by nuclear extract of the cycloheximide-treated regenerating liver showed the same results with normal liver. These results suggest that transcriptional regulation of H2B histone gene during liver regeneration may be mediated by nuclear factors that are newly induced by partial hepatectomy.

摘要

我们通过DNA酶I足迹分析，研究了大鼠H2B组蛋白基因上游区域的DNA合成、H2B组蛋白mRNA水平以及核蛋白与各种元件的结合模式。部分肝切除术后，DNA合成和H2B组蛋白mRNA水平均升高，分别在术后24小时和36小时达到最大刺激。在DNA酶I足迹分析中，部分肝切除术后H2B组蛋白mRNA水平最大增加时，需要与三个元件相互作用的核因子，即位于-19bp的TATA（位点AR）、位于-29bp的位点B和位于-69bp的CAAT（位点C）。环己酰亚胺处理的再生肝核提取物的DNA酶I保护模式与正常肝相同。这些结果表明，肝再生过程中H2B组蛋白基因的转录调控可能由部分肝切除新诱导的核因子介导。