Giambanco I, Verzini M, Donato R
Section of Anatomy, Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy.
Biochem Biophys Res Commun. 1993 Nov 15;196(3):1221-6. doi: 10.1006/bbrc.1993.2382.
Annexins V and VI, two Ca(2+)-dependent phospholipid- and membrane-binding proteins, were immunochemically measured in a number of rat organs and tissues during post-natal development. Annexin V proved much more abundant than annexin VI irrespective of the organ and the post-natal period considered. In the brain, annexin V accumulated at a high rate from the end of the first post-natal week onward, whereas annexin VI was expressed in extremely low amounts irrespective of the period investigated. In contrast, the levels of both annexins in the heart were nearly constant, in the post-natal period investigated. In skeletal muscles, annexin V and VI levels were high around post-natal day 1 and decreased thereafter. A similar pattern was observed for annexin V in liver, whereas the amounts of annexin VI in this organ were at the limits of detectability. In the lung, annexin V accumulated almost linearly from birth to adulthood, whereas annexin VI was relatively high at birth, decreased to low levels by the end of the first post-natal week, and re-accumulated thereafter. Among the organs examined, the lung and heart proved the richest sources of annexins V and VI. Annexin V appears to be a useful marker of and to be implicated in brain, lung and skeletal muscle maturation.
膜联蛋白V和VI是两种依赖钙离子的磷脂和膜结合蛋白，在出生后发育过程中对大鼠的多个器官和组织进行了免疫化学测定。无论所考虑的器官和出生后时期如何，膜联蛋白V都比膜联蛋白VI丰富得多。在大脑中，从出生后第一周结束起，膜联蛋白V就以高速率积累，而无论所研究的时期如何，膜联蛋白VI的表达量都极低。相比之下，在所研究的出生后时期，心脏中两种膜联蛋白的水平几乎恒定。在骨骼肌中，出生后第1天左右膜联蛋白V和VI的水平较高，此后下降。肝脏中的膜联蛋白V也观察到类似模式，而该器官中膜联蛋白VI的量处于可检测的极限。在肺中，膜联蛋白V从出生到成年几乎呈线性积累，而膜联蛋白VI在出生时相对较高，在出生后第一周结束时降至低水平，此后再次积累。在所检查的器官中，肺和心脏被证明是膜联蛋白V和VI最丰富的来源。膜联蛋白V似乎是大脑、肺和骨骼肌成熟的有用标志物，并与之相关。
