Römer S, Hugueney P, Bouvier F, Camara B, Kuntz M
Institut de Biologie Moléculaire des Plantes du C.N.R.S., Université Louis Pasteur, Strasbourg, France.
Biochem Biophys Res Commun. 1993 Nov 15;196(3):1414-21. doi: 10.1006/bbrc.1993.2410.
We have shown that both geranylgeranyl pyrophosphate synthase and phytoene synthase from pepper and tomato chromoplasts are very similar in terms of enzymic activity and immunological properties. This enabled us to clone cDNAs specific for phytoene synthase from ripening tomato and pepper fruits and to compare their amino acid sequences with those of bacterial phytoene synthase and yeast squalene synthase. Comparison of the nucleotide sequence of the 3'-untranslated region of the pepper phytoene synthase cDNA suggests that this region was subjected to complex recombination events. RNA gel blot hybridizations revealed that induction of phytoene synthase gene expression is much stronger in tomato than in pepper fruits. In contrast with tomato, 2 phytoene synthase transcripts of different size are present in pepper leaves and fruits. Comparison of the expression pattern of the genes encoding geranylgeranyl pyrophosphate synthase, phytoene synthase and phytoene desaturase revealed that these genes are not co-regulated during pepper fruit ripening.
我们已经表明，辣椒和番茄有色体中的香叶基香叶基焦磷酸合酶和八氢番茄红素合酶在酶活性和免疫学特性方面非常相似。这使我们能够从成熟的番茄和辣椒果实中克隆出八氢番茄红素合酶特异性的cDNA，并将它们的氨基酸序列与细菌八氢番茄红素合酶和酵母鲨烯合酶的氨基酸序列进行比较。辣椒八氢番茄红素合酶cDNA 3'-非翻译区核苷酸序列的比较表明，该区域经历了复杂的重组事件。RNA凝胶印迹杂交显示，八氢番茄红素合酶基因表达的诱导在番茄中比在辣椒果实中要强得多。与番茄不同，辣椒叶片和果实中存在两种不同大小的八氢番茄红素合酶转录本。对编码香叶基香叶基焦磷酸合酶、八氢番茄红素合酶和八氢番茄红素去饱和酶的基因表达模式的比较表明，这些基因在辣椒果实成熟过程中不是共同调控的。
