Woolf A S, Fine L G
Department of Medicine, University College and Middlesex School of Medicine, London, UK.
Pediatr Nephrol. 1993 Oct;7(5):605-8. doi: 10.1007/BF00852565.
We review the available methods of creating genetically engineered kidneys. These include transgenic technology to introduce novel genes or delete existing genes and methods of gene transfer into the post-natal or adult kidney. The use of such technology has provided insights into renal development and growth and created new animal models of human diseases. Although some of these techniques are of potential use for introducing therapeutically useful gene products into the diseased kidney, many problems remain to be solved before this aim is attained.
我们回顾了创建基因工程肾脏的现有方法。这些方法包括引入新基因或删除现有基因的转基因技术，以及将基因转移到出生后或成年肾脏的方法。此类技术的应用为肾脏发育和生长提供了见解，并创建了人类疾病的新动物模型。尽管其中一些技术在将治疗性有用基因产物引入患病肾脏方面具有潜在用途，但在实现这一目标之前仍有许多问题有待解决。
