Qu B, Zhuo Z, Yi Y, Feng G, Zheng J, Liang Q, Li Y
Cancer center, Sun Yat-Sen University of medical Sciences, Guangzhou, China.
Yan Ke Xue Bao. 1993 Mar;9(1):38-9, 37.
G-banding and karyotype analyses of cells in seventeen passages of SO-Rb50 during a long period of culture for about four years were performed. Three chromosome markers 13q14-, 1p36+ and 12p13+ were found. Cells possessed 13q14- reduced to zero after the 200th passage while 1p+ and 12p+ cells increased to 100% after 30 and 200 passages respectively. Abnormal chromosomes, ring chromosomes, chromosome radiuses and double minutes were also observed. These chromosomal changes were more often seen before the 200th passage. The significance of these changes are discussed.
对SO-Rb50细胞在约四年的长期培养过程中的十七代细胞进行了G显带和核型分析。发现了三个染色体标记13q14-、1p36+和12p13+。细胞在第200代后13q14-减少至零，而1p+和12p+细胞分别在第30代和第200代后增加到100%。还观察到异常染色体、环状染色体、染色体半径和双微体。这些染色体变化在第200代之前更常出现。讨论了这些变化的意义。
