一种与细胞周期蛋白依赖性激酶2相关的激酶PSSALRE/cdk5与微管相关的脯氨酸定向蛋白激酶——tau蛋白激酶II的30 kDa亚基同源。
A cdc2-related kinase PSSALRE/cdk5 is homologous with the 30 kDa subunit of tau protein kinase II, a proline-directed protein kinase associated with microtubule.
作者信息
Kobayashi S, Ishiguro K, Omori A, Takamatsu M, Arioka M, Imahori K, Uchida T
机构信息
Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
出版信息
FEBS Lett. 1993 Dec 6;335(2):171-5. doi: 10.1016/0014-5793(93)80723-8.
We previously reported that tau protein kinase II (TPKII) from bovine brain was composed of 30 kDa and 23 kDa subunits. The 30 kDa subunit of TPKII can be regarded as a catalytic subunit because of its ATP-binding activity. Antibodies directed against TPKII-phosphorylated tau also reacted with tau phosphorylated by cdc2 kinase obtained from starfish oocytes, indicating that TPKII and cdc2 kinase phosphorylate the same sites. We determined the amino acid sequence of the 30 kDa subunit and found it to be homologous with a cdc2-related kinase, PSSALRE/cdk5. Moreover, an antibody against PSSALRE/cdk5 reacted with the 30 kDa subunit. These results indicate that the 30 kDa subunit of TPKII is bovine homologue of PSSALRE/cdk5. Expression of the 30 kDa subunit mRNA was enhanced in juvenile rat brain. This result supports our previous hypothesis that the kinase works actively in juvenile brain.
我们之前报道过，来自牛脑的tau蛋白激酶II（TPKII）由30 kDa和23 kDa的亚基组成。TPKII的30 kDa亚基因其ATP结合活性可被视为催化亚基。针对TPKII磷酸化tau的抗体也与海星卵母细胞来源的cdc2激酶磷酸化的tau发生反应，表明TPKII和cdc2激酶磷酸化相同的位点。我们测定了30 kDa亚基的氨基酸序列，发现它与一种cdc2相关激酶PSSALRE/cdk5同源。此外，针对PSSALRE/cdk5的抗体与30 kDa亚基发生反应。这些结果表明，TPKII的30 kDa亚基是PSSALRE/cdk5的牛同源物。30 kDa亚基mRNA在幼年大鼠脑中的表达增强。这一结果支持了我们之前的假设，即该激酶在幼脑中活跃发挥作用。
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