Girard L R, Castle A M, Hand A R, Castle J D, Mirels L
Biology Department, University of California, San Diego, La Jolla 92093-0322.
J Biol Chem. 1993 Dec 15;268(35):26592-601.
We have isolated and characterized cDNA clones derived from a developmentally regulated neonatal rat submandibular gland salivary protein gene called "common salivary protein 1" (CSP1). Identical clones were also identified in cDNA libraries from adult male parotid, submandibular, and sublingual glands. CSP1 transcripts are at least 10-fold more abundant in the sublingual gland than in the submandibular or parotid glands. In situ hybridization and immunocytochemical localization demonstrated the presence of CSP1 transcripts and proteins in sublingual gland serous demilune cells, parotid and submandibular gland intercalated duct cells, and in the type III (proacinar) cells of the neonatal submandibular gland. This cell-type distribution is similar to that described by Ball and colleagues (Ball, W. D., Hand, A. R., and Johnson, A. O. (1988) Dev. Biol. 125, 265-279) for the developmentally regulated submandibular gland B1-immunoreactive proteins. Immunoblotting of salivary secretion identified proteins of M(r) 20,000 in sublingual, 16,000 in submandibular and 22,000 and 16,000 in parotid gland. The M(r) 20,000 sublingual and 22,000 parotid proteins represent N-glycosylated forms of a M(r) 16,000 apoprotein, suggesting that these salivary proteins arise by post-translational modification of a common precursor.
我们已经分离并鉴定了来自发育调控的新生大鼠下颌下腺唾液蛋白基因“普通唾液蛋白1”（CSP1）的cDNA克隆。在成年雄性腮腺、下颌下腺和舌下腺的cDNA文库中也鉴定出了相同的克隆。CSP1转录本在舌下腺中的丰度至少比下颌下腺或腮腺高10倍。原位杂交和免疫细胞化学定位显示，CSP1转录本和蛋白存在于舌下腺浆液半月细胞、腮腺和下颌下腺闰管细胞以及新生大鼠下颌下腺的III型（前腺泡）细胞中。这种细胞类型分布与Ball及其同事（Ball, W. D., Hand, A. R., and Johnson, A. O. (1988) Dev. Biol. 125, 265 - 279）描述的发育调控的下颌下腺B1免疫反应性蛋白相似。唾液分泌的免疫印迹分析在舌下腺中鉴定出分子量为20,000的蛋白，在下颌下腺中为16,000，在腮腺中为22,000和16,000。舌下腺中分子量为20,000的蛋白和腮腺中分子量为22,000的蛋白代表分子量为16,000的载脂蛋白的N - 糖基化形式，这表明这些唾液蛋白是由共同前体的翻译后修饰产生的。
