巴豆毒蛋白II的初步晶体学研究。

Preliminary crystallographic studies of crotin II.

作者信息

Chen M H, Zhou K J, Fu Z J, Pan K Z

机构信息

Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou.

出版信息

J Mol Biol. 1993 Dec 5;234(3):908-9. doi: 10.1006/jmbi.1993.1641.

DOI:10.1006/jmbi.1993.1641

PMID:8254687

Abstract

Crotin II isolated from the seeds of Euphorbiacea Croton tiglium has been crystallized. Crystals were grown by vapor diffusion using KCl as the precipitant. The crystal of crotin II belongs to the space group P6(1) or P6(5) with cell parameters a = b = 94.62 A, c = 28.43 A, alpha = beta = 90 degrees, gamma = 120 degrees. The asymmetric unit contains one molecule of 15,000 Da. A data set to 1.82 A has been collected on an area detector.

摘要

从大戟科巴豆属植物巴豆的种子中分离得到的巴豆毒素II已经结晶。通过以氯化钾作为沉淀剂的气相扩散法培养晶体。巴豆毒素II的晶体属于空间群P6(1)或P6(5)，晶胞参数为a = b = 94.62 Å，c = 28.43 Å，α = β = 90°，γ = 120°。不对称单元包含一个分子量为15,000道尔顿的分子。已在面积探测器上收集了分辨率为1.82 Å的数据集。