玉米幼苗磷脂转移蛋白的结晶及初步X射线晶体学分析

Crystallization and preliminary X-ray crystallographic analysis of phospholipid transfer protein from maize seedlings.

作者信息

Shin D H, Hwang K Y, Kim K K, Kim S, Sweet R M, Suh S W

机构信息

Department of Chemistry, College of Natural Sciences, Seoul National University, Korea.

出版信息

Proteins. 1994 May;19(1):80-3. doi: 10.1002/prot.340190111.

DOI:10.1002/prot.340190111

PMID:8066090

Abstract

Phospholipid transfer protein from maize seedlings has been crystallized using trisodium citrate as precipitant. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1) with unit cell dimensions of a = 24.46 A, b = 49.97 A, and c = 69.99 A. The presence of one molecule in the asymmetric unit gives a crystal volume per protein mass (Vm) of 2.36 A3/Da and a solvent content of 48% by volume. The X-ray diffraction pattern extends at least to 1.6 A Bragg spacing when exposed to both CuK alpha and synchrotoron X-rays. A set of X-ray data to approximately 1.9 A Bragg spacing has been collected from a native crystal.

摘要

已使用柠檬酸三钠作为沉淀剂使来自玉米幼苗的磷脂转移蛋白结晶。该晶体属于正交晶系空间群P2(1)2(1)2(1)，晶胞参数为a = 24.46 Å，b = 49.97 Å，c = 69.99 Å。不对称单元中存在一个分子，使得每蛋白质质量的晶体体积（Vm）为2.36 Å3/Da，溶剂含量为48%（体积）。当用CuKα和同步加速器X射线照射时，X射线衍射图谱至少延伸到1.6 Å的布拉格间距。已从天然晶体收集了一组约1.9 Å布拉格间距的X射线数据。