完整红细胞的31P核磁共振谱：尿苷二磷酸葡萄糖和尿苷二磷酸半乳糖的定量分析

31P NMR spectra of intact red blood cells: quantitation of UDPGlucose and UDPGalactose.

作者信息

Wehrli S L, Palmieri M J, Reynolds R A, Segal S

机构信息

Department of Pediatrics, University of Pennsylvania School of Medicine.

出版信息

Magn Reson Med. 1993 Oct;30(4):494-7. doi: 10.1002/mrm.1910300413.

DOI:10.1002/mrm.1910300413

PMID:8255198

Abstract

The combined levels of uridine diphosphogalactose (UDPGal) and uridine diphosphoglucose (UDPGlu) were measured directly by 31P NMR spectroscopy in intact fully oxygenated erythrocytes. Quantitative analysis was obtained using a sealed capillary of methylene diphosphonate (MDP) calibrated with standard solutions of UDPGlu and UDPGal of known concentration prepared in KRB/BSA. The combined peaks of UDPGlu and UDPGal were integrated after subtraction of the underlying broad component originating from membrane phospholipids. The average concentration of 27.16 +/- 5.18 nmole/ml or 8.08 +/- 1.36 mumole/100 g hemoglobin obtained for these metabolites correlated well with their total determined by HPLC of trichloracetic acid (TCA) extracts of the same samples.

摘要

在完整的完全氧合红细胞中，通过31P核磁共振光谱法直接测量尿苷二磷酸半乳糖（UDPGal）和尿苷二磷酸葡萄糖（UDPGlu）的联合水平。使用密封的亚甲基二膦酸盐（MDP）毛细管进行定量分析，该毛细管由在KRB/BSA中制备的已知浓度的UDPGlu和UDPGal标准溶液校准。在减去源自膜磷脂的潜在宽峰成分后，对UDPGlu和UDPGal的联合峰进行积分。这些代谢物的平均浓度为27.16±5.18纳摩尔/毫升或8.08±1.36微摩尔/100克血红蛋白，与通过相同样品的三氯乙酸（TCA）提取物的高效液相色谱法测定的总量相关性良好。