地中海伞藻的叶绿体膜。II. 叶绿体膜的拓扑结构。
Chloroplast membranes of the green alga Acetabularia mediterranea. II. Topography of the chloroplast membrane.
作者信息
Apel K, Miller K R, Bogorad L, Miller G J
出版信息
J Cell Biol. 1976 Dec;71(3):876-93. doi: 10.1083/jcb.71.3.876.
The localization of the chlorophyll-protein complexes inside the thylakoid membrane of Acetabularia mediterranea was determined by fractionating the chloroplast membrane with EDTA and Triton X-100, by using pronase treatment, and by labeling the surface-exposed proteins with 125I. The effects of the various treatments were established by electrophoresis of the solubilized membrane fractions and electron microscopy. After EDTA and pronase treatment, the membrane structure was still intact. Only the two chlorophyll-protein complexes of 67,000 and 152,000 daltons and an additional polypeptides were found in the membrane before the EDTA and pronase treatment. The 125,000 dalton complex seems to be buried inside the lipid layer. The 23,000 dalton subunit of the 67,000 dalton complex is largely exposed to the surface of the EDTA-insoluble membrane and only the chlorophyll-binding subunit of 21,500 daltons is buried inside the lipid layer.
通过用乙二胺四乙酸（EDTA）和曲拉通X-100对叶绿体膜进行分级分离、使用链霉蛋白酶处理以及用¹²⁵I标记表面暴露的蛋白质，确定了地中海伞藻类囊体膜内叶绿素-蛋白质复合物的定位。通过对溶解的膜组分进行电泳和电子显微镜观察，确定了各种处理的效果。经EDTA和链霉蛋白酶处理后，膜结构仍然完整。在进行EDTA和链霉蛋白酶处理之前，在膜中仅发现了67,000道尔顿和152,000道尔顿的两种叶绿素-蛋白质复合物以及一种额外的多肽。125,000道尔顿的复合物似乎埋在脂质层内部。67,000道尔顿复合物的23,000道尔顿亚基大部分暴露于EDTA不溶性膜的表面，只有21,500道尔顿的叶绿素结合亚基埋在脂质层内部。
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