Institut für Blochemie der Universität Bern, Freiestrasse 3, 3012, Bern, Switzerland.
Photosynth Res. 1981 Sep;2(3):203-12. doi: 10.1007/BF00032359.
Upon protoolytle treatment of thylakoid membranes, extrinsic proteins are selectively degraded. The remaining resistant proteins have been analyzed by SDS-PAGE. In the thylakoids of Chlamydomonas reinhardii, six polypeptides or protein fragments of 20 kD or higher are resistant to proteolysis. These intrinsic proteins have been identified as: the apoproteins of the chlorophyll-protein complexes CP I and LHCP; a polypeptide whose presence is related to the chlorophyll b content of the cells; and a portion of a chloroplast-made 34 kD polypeptide. Furthermore, after proteolytic treatment of the membranes, the LHCP complex can be resolved into two subcomplexes, apparently differing in their polypeptide composition.
经蛋白水解处理后，类囊体膜中的外向蛋白被选择性降解。剩余的抗性蛋白已通过 SDS-PAGE 进行分析。在莱茵衣藻的类囊体中，有六种 20kD 或更高的多肽或蛋白片段对蛋白水解具有抗性。这些内在蛋白已被鉴定为：叶绿素蛋白复合物 CP I 和 LHCP 的脱辅基蛋白；一种与细胞叶绿素 b 含量有关的多肽；以及叶绿体合成的 34kD 多肽的一部分。此外，在膜的蛋白水解处理后，LHCP 复合物可以分解为两个亚复合物，其多肽组成显然不同。
