Smith T J
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907.
Methods Cell Biol. 1993;37:75-93. doi: 10.1016/s0091-679x(08)60244-7.
Although monoclonal antibodies can have different properties, it should still be possible to purify any antibody to homogeneity. Often, by utilizing these differences, the purification procedure can be improved. All of the methods discussed are fairly simple and fast. With 1 week of work, over 50 mg of purified antibody can be realized. These methods can also yield material of sufficient quality for structural studies.
尽管单克隆抗体可能具有不同的特性,但仍应能够将任何抗体纯化至均一性。通常,利用这些差异可以改进纯化程序。所讨论的所有方法都相当简单且快速。经过一周的工作,可以获得超过50毫克的纯化抗体。这些方法还可以产生质量足以用于结构研究的材料。