Choy B, Green M R
Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester 01605.
Nature. 1993 Dec 9;366(6455):531-6. doi: 10.1038/366531a0.
Eukaryotic activator proteins (activators) stimulate transcription by increasing assembly of the preinitiation complex. We have developed methods to quantify the stable assembly of general transcription factors into transcriptional complexes in response to activators. We show that activators function during at least two stages of preinitiation complex assembly: first, to recruit the general transcription factor TFIIB, and then at a second step, after TFIIB entry. It is at this second step that the TATA-box binding protein associated factors act. This step also seems to be critical for activators to stimulate transcription synergistically.
真核激活蛋白（激活剂）通过增加起始前复合物的组装来刺激转录。我们已经开发出方法来量化通用转录因子响应激活剂而稳定组装到转录复合物中的情况。我们表明，激活剂在起始前复合物组装的至少两个阶段发挥作用：首先，招募通用转录因子TFIIB，然后在第二步，在TFIIB进入之后。正是在这第二步中，TATA框结合蛋白相关因子发挥作用。这一步骤似乎对于激活剂协同刺激转录也至关重要。
