Tsukada N, Matsuda M, Miyagi K, Yanagisawa N
Health Medical Center, Shinshu University, Matsumoto, Japan.
Neurology. 1993 Dec;43(12):2679-82. doi: 10.1212/wnl.43.12.2679.
We investigated the presence of soluble intercellular adhesion molecule-1 (sICAM-1) and soluble tumor necrosis factor receptor (sTNF-R) antigens in the CSF of patients with multiple sclerosis (MS) using a double-determinant ELISA. Patients with acute relapsing MS during an exacerbation (p < 0.001) and those with chronic progressive MS (p < 0.001) had significantly increased CSF levels of sICAM-1 compared with subjects with other neurologic diseases. CSF levels of sTNF-R were also significantly increased in patients with acute relapsing MS during an exacerbation (p < 0.001) and chronic progressive MS (p < 0.001) compared with subjects with other neurologic diseases. CSF levels of sICAM-1 and sTNF-R were positively correlated in patients with acute relapsing MS during an exacerbation (r = 0.81, p < 0.01) and chronic progressive MS (r = 0.86, p < 0.001). These results suggest that active immune reactions involving ICAM-1 and TNF-R production are present within the CNS and that both sICAM-1 and sTNF-R are important immunologic markers of the clinical activity of MS.
我们使用双检测酶联免疫吸附测定法（ELISA）研究了多发性硬化症（MS）患者脑脊液中可溶性细胞间黏附分子-1（sICAM-1）和可溶性肿瘤坏死因子受体（sTNF-R）抗原的存在情况。与患有其他神经系统疾病的受试者相比，处于病情加重期的急性复发型MS患者（p < 0.001）和慢性进展型MS患者（p < 0.001）脑脊液中的sICAM-1水平显著升高。与患有其他神经系统疾病的受试者相比，处于病情加重期的急性复发型MS患者（p < 0.001）和慢性进展型MS患者（p < 0.001）脑脊液中的sTNF-R水平也显著升高。在处于病情加重期的急性复发型MS患者（r = 0.81，p < 0.01）和慢性进展型MS患者（r = 0.86，p < 0.001）中，脑脊液中的sICAM-1水平和sTNF-R水平呈正相关。这些结果表明，中枢神经系统内存在涉及ICAM-1和TNF-R产生的活跃免疫反应，并且sICAM-1和sTNF-R都是MS临床活动的重要免疫标志物。
