Childs G V, Cole D E, Kubek M, Tobin R B, Wilber J F
J Histochem Cytochem. 1978 Nov;26(11):901-8. doi: 10.1177/26.11.82570.
Thyrotropin-releasing hormone (TRH) immunoreactivity was localized in the rat anterior pituitary with rabbit anti-TRH sera and the unlabeled antibody peroxidase-antiperoxidase complex (PAP) technique. Stain was present in secretory granules of cells possessing morphological characteristics of thyrotropes, gonadotropes and lactotropes. Antibody absorption studies with anti-TRH sera absorbed with TRH, 3 diastereoisomeric analogues of TRH, gonadotropin-releasing hormone (GnRH), bovine serum albumin, thyrotropin, prolactin, adrenocorticotropin, luteinizing hormone, follicle stimulating hormone were performed to determine the specificity of the staining reaction. Only absorption with TRH resulted in a significant reduction in staining intensity. In vitro experiments were then begun with hemipituitaries to ascertain if intrapituitary TRH might originate by sequestration of exogenous, plasma membrane bound TRH or by de novo synthesis. The results suggest that anterior pituitary TRH is of endogenous origin.
采用兔抗促甲状腺激素释放激素（TRH）血清和未标记抗体过氧化物酶-抗过氧化物酶复合物（PAP）技术，对大鼠垂体前叶中TRH免疫反应性进行定位。染色出现在具有促甲状腺细胞、促性腺细胞和催乳细胞形态特征的细胞分泌颗粒中。用TRH、TRH的3种非对映异构体类似物、促性腺激素释放激素（GnRH）、牛血清白蛋白、促甲状腺激素、催乳素、促肾上腺皮质激素、黄体生成素、卵泡刺激素吸收抗TRH血清进行抗体吸收研究，以确定染色反应的特异性。只有用TRH吸收会导致染色强度显著降低。然后开始用垂体半叶进行体外实验，以确定垂体中的TRH是否可能通过隔离外源性、与质膜结合的TRH或从头合成产生。结果表明垂体前叶TRH起源于内源性。
