延髓头端腹外侧区在对中缝隐核刺激的升压反应中的作用。

Role of the rostral ventrolateral medulla in the pressor response to stimulation of the nucleus raphe obscurus.

Campos Júnior R R, Futuro-Neto H A, Guertzenstein P G

Departamento de Fisiologia, Escola Paulista de Medicina, São Paulo, Brasil.

Braz J Med Biol Res. 1993 Jun;26(6):623-31.

Abstract

Electrical stimulation of the nucleus raphe obscurus (NRO) in urethane-anesthetized rats increases arterial blood pressure (BP) between 20 and 95 mmHg (mean, 61.14 +/- 6.57; N = 30). 2. Unilateral electrolytic destruction of the rostral ventrolateral medulla (RVLM) did not reduce BP or heart rate (HR) but significantly reduced the pressor response to NRO stimulation (control, delta 76.0 +/- 5.4 mmHg; after lesion, delta 26.0 +/- 13.9 mmHg; P < 0.01, N = 5). 3. Bilateral destruction of the RVLM reduced basal BP (control, 104.1 +/- 11.4 mmHg; after lesion, 58.0 +/- 5.7 mmHg; P < 0.01) and the pressor response to NRO stimulation (control, delta 71.6 +/- 7.3; after lesion, delta 12.5 +/- 3.8 mmHg; P < 0.01, N = 6). 4. When topically applied to or microinjected into the RVLM, pentobarbital sodium (200 nl/1 microliters, 10 nmol) decreased BP, HR and the pressor response to NRO stimulation (control, delta 56.2 +/- 6.7 mmHg; after pentobarbital, delta 11.2 +/- 3.1 mmHg; P < 0.01, N = 13). Similar effects were obtained when glycine (200 nl, 50 nmol) was microinjected into RVLM (control, delta 40.5 +/- 5.9 mmHg; after glycine, delta 18.1 +/- 4.9 mmHg; P < 0.01, N = 6). 5. We conclude that RVLM is essential for the pressor response to NRO stimulation.

摘要

对氨基甲酸乙酯麻醉的大鼠中缝隐核（NRO）进行电刺激，可使动脉血压（BP）在20至95 mmHg之间升高（平均值为61.14±6.57；N = 30）。2. 对延髓头端腹外侧区（RVLM）进行单侧电解毁损，并未降低血压或心率（HR），但显著降低了对NRO刺激的升压反应（对照组，Δ76.0±5.4 mmHg；毁损后，Δ26.0±13.9 mmHg；P < 0.01，N = 5）。3. 对RVLM进行双侧毁损降低了基础血压（对照组，104.1±11.4 mmHg；毁损后，58.0±5.7 mmHg；P < 0.01）以及对NRO刺激的升压反应（对照组，Δ71.6±7.3；毁损后，Δ12.5±3.8 mmHg；P < 0.01，N = 6）。4. 当将戊巴比妥钠（200 nl/1微升，10 nmol）局部应用于或微量注射到RVLM时，可降低血压、心率以及对NRO刺激的升压反应（对照组，Δ56.2±6.7 mmHg；注射戊巴比妥后，Δ11.2±3.1 mmHg；P < 0.01，N = 13）。当将甘氨酸（200 nl，50 nmol）微量注射到RVLM时也获得了类似的效果（对照组，Δ40.5±5.9 mmHg；注射甘氨酸后，Δ18.1±4.9 mmHg；P < 0.01，N = 6）。5. 我们得出结论，RVLM对于对NRO刺激的升压反应至关重要。