Ning Z Q, Murphy J J
Division of Life Sciences, King's College London, GB.
Eur J Immunol. 1993 Dec;23(12):3369-72. doi: 10.1002/eji.1830231247.
We have investigated the effects of calcium ionophore on apoptosis of Ramos human B cells. Our results show that the calcium ionophore A23187 at defined concentrations leads to apoptosis of Ramos cells. The majority of cells (> 90%) undergo apoptosis in response to ionophore. The response is rapid and nuclear condensation and DNA degradation can be detected within 2 h after addition of ionophore. In attempts to define the changes in gene expression preceding apoptosis, we investigated the expression of a panel of early response genes in these cells after ionophore addition. We show that calcium ionophore-induced apoptosis of Ramos cells is preceded by the induced expression of a number of early response genes. These results are consistent with calcium ionophore initiating changes in gene expression which may be important in signaling these cells to undergo apoptosis.
我们研究了钙离子载体对Ramos人B细胞凋亡的影响。我们的结果表明，特定浓度的钙离子载体A23187可导致Ramos细胞凋亡。大多数细胞（>90%）对离子载体产生反应而发生凋亡。这种反应迅速，在添加离子载体后2小时内即可检测到核浓缩和DNA降解。为了确定凋亡前基因表达的变化，我们研究了添加离子载体后这些细胞中一组早期反应基因的表达。我们表明，钙离子载体诱导的Ramos细胞凋亡之前，有许多早期反应基因的诱导表达。这些结果与钙离子载体引发基因表达变化一致，这可能对这些细胞发生凋亡的信号传导很重要。
