ten Berge P J, Danen E H, van Muijen G N, Jager M J, Ruiter D J
Department of Pathology, University Hospital Nijmegen, The Netherlands.
Invest Ophthalmol Vis Sci. 1993 Dec;34(13):3635-40.
During the process of metastasis, changes in cell-cell and cell-matrix contacts occur; therefore, expression of integrins, a superfamily of adhesion molecules, may be important. Expression of integrins has been studied extensively in cutaneous melanoma. Because it is known that uveal melanoma has a metastatic behavior different from cutaneous melanoma, the authors investigated integrin expression in uveal melanoma.
The authors used monoclonal antibodies recognizing integrin subunits alpha 1-6, alpha v, beta 1, and beta 4 and integrins alpha v beta 3 and alpha v beta 5 on frozen sections of 32 human primary uveal melanomas and 4 metastases, followed by an avidin-biotin-peroxidase complex-immunoperoxidase technique.
As in cutaneous melanoma, alpha 4 expression was rare, but most lesions expressed alpha 3 and alpha 6. In contrast to cutaneous melanoma, in which alpha 2 is well expressed in most lesions and alpha 5 is expressed only in a small percentage of lesions, alpha 2 expression was rare in uveal melanoma and alpha 5 expression was found in all lesions. A major difference was observed with regard to the alpha v beta 3 vitronectin receptor. In contrast to cutaneous melanoma, in which alpha v beta 3 is expressed in advanced primary melanomas and metastases, alpha v beta 3 was not detected in any of the primary uveal melanomas, but all lesions strongly expressed alpha v beta 5.
Integrin expression in uveal melanoma cannot be correlated with cell type or invasiveness. In contrast to cutaneous melanoma, it seems that determination of the integrin expression profile is not suitable for categorizing uveal melanomas as less malignant and highly malignant lesions.
在转移过程中，细胞间和细胞与基质的接触会发生变化；因此，整合素（一种黏附分子超家族）的表达可能很重要。整合素的表达已在皮肤黑色素瘤中得到广泛研究。由于已知葡萄膜黑色素瘤具有与皮肤黑色素瘤不同的转移行为，作者研究了葡萄膜黑色素瘤中整合素的表达。
作者使用识别整合素亚基α1 - 6、αv、β1和β4以及整合素αvβ3和αvβ5的单克隆抗体，对32例人类原发性葡萄膜黑色素瘤和4例转移灶的冰冻切片进行检测，随后采用抗生物素蛋白 - 生物素 - 过氧化物酶复合物免疫过氧化物酶技术。
与皮肤黑色素瘤一样，α4表达罕见，但大多数病变表达α3和α6。与皮肤黑色素瘤不同，在皮肤黑色素瘤中大多数病变α2表达良好，α5仅在一小部分病变中表达，而在葡萄膜黑色素瘤中α2表达罕见，且在所有病变中均发现α5表达。在αvβ3玻连蛋白受体方面观察到一个主要差异。与皮肤黑色素瘤不同，在皮肤黑色素瘤中αvβ3在晚期原发性黑色素瘤和转移灶中表达，而在任何原发性葡萄膜黑色素瘤中均未检测到αvβ3，但所有病变均强烈表达αvβ5。
葡萄膜黑色素瘤中的整合素表达与细胞类型或侵袭性无关。与皮肤黑色素瘤不同，似乎整合素表达谱的测定不适用于将葡萄膜黑色素瘤分类为低恶性和高恶性病变。
