通过免疫吸附色谱法纯化α-2巨球蛋白

Purification of alpha-2 macroglobulin by immunoadsorbent chromatography.

作者信息

McEntire J E

出版信息

J Immunol Methods. 1978;24(1-2):39-45. doi: 10.1016/0022-1759(78)90084-4.

Abstract

A method for preparation of alpha-2 macroglobulin (alpha2M) by immunoadsorbent chromatography utilizing rabbit--anti-human alpha2M-conjugated Sepharose 4B is described. This procedure offers a rapid, simple and inexpensive method for purification of alpha2M from as little as 2.0 ml of human serum. By multiple applications to the absorbent column, as much as 10 mg of purified alpha2M may be easily prepared in one day. This technique is especially useful for isolation of alpha2M from individual sera and for studies of cultured cell supernatants containing human alpha2M.

摘要

描述了一种利用兔抗人α2M偶联的琼脂糖凝胶4B通过免疫吸附色谱法制备α2-巨球蛋白（α2M）的方法。该方法为从低至2.0毫升人血清中纯化α2M提供了一种快速、简单且廉价的方法。通过多次应用于吸附柱，一天内可轻松制备多达10毫克的纯化α2M。该技术对于从个体血清中分离α2M以及研究含有人α2M的培养细胞上清液特别有用。