Rolin S, Paindavoine P, Hanocq-Quertier J, Hanocq F, Claes Y, Le Ray D, Overath P, Pays E
Department of Molecular Biology, Free University of Brussels, Rhode Saint Genèse, Belgium.
Mol Biochem Parasitol. 1993 Sep;61(1):115-25. doi: 10.1016/0166-6851(93)90164-s.
Pleomorphic bloodstream forms of Trypanosoma brucei differentiate synchronously into procyclic forms when cultivated at 27 degrees C in the presence of citrate/cis-aconitate. The activity of adenylate cyclase was monitored during this process. Two phases of transient stimulation were observed. The first phase occurred 6-10 h after the triggering of differentiation, a period which immediately follows the release of the bulk of the VSG and immediately precedes both the first cell division and the loss of the bloodstream-specific ESAG 4 transmembrane adenylate cyclase. The second phase occurred between 20 and 40 h, when the cells that emerged from the first division began to proliferate. These observations suggest that cAMP may be involved in differentiation/proliferation of the parasite.
当在27摄氏度、存在柠檬酸盐/顺乌头酸的条件下培养时，布氏锥虫的多形血流形式会同步分化为前循环形式。在此过程中监测了腺苷酸环化酶的活性。观察到两个短暂刺激阶段。第一阶段发生在分化触发后6 - 10小时，这一时期紧接着大部分VSG的释放，且紧接在第一次细胞分裂和血流特异性ESAG 4跨膜腺苷酸环化酶丧失之前。第二阶段发生在20至40小时之间，此时从第一次分裂中出现的细胞开始增殖。这些观察结果表明，cAMP可能参与了寄生虫的分化/增殖。
