血液多形核白细胞在尿液中孵育后白三烯B4的生成

Leukotriene B4 generation by blood polymorphonuclear leukocytes after incubation in urine.

作者信息

Shimizu T, Jodal U, Strandvik B

机构信息

Department of Paediatrics, University of Göteborg, Sweden.

出版信息

Prostaglandins Leukot Essent Fatty Acids. 1993 Oct;49(4):761-4. doi: 10.1016/0952-3278(93)90022-o.

DOI:10.1016/0952-3278(93)90022-o

PMID:8259372

Abstract

To investigate the capacity of leukocytes in urine to produce leukotriene B4 (LTB4), we evaluated calcium ionophore A23187-induced LTB4 generation by human blood polymorphonuclear leukocytes (PMNL) after these cells were incubated in urine under various conditions. LTB4 production was markedly reduced after incubation of PMNL in urine of high or low pH and high osmolarity. The production was also inhibited by a prolonged incubation period. There was a significant correlation (r = 0.82, p < 0.01) between LTB4 production and PMNL viability. Our results indicate that it is possible to evaluate the LTB4 production capacity of urinary PMNL by determining PMNL viability only. Furthermore acidification of urine hamper the LTB4 generation which might be unfavorable in vivo.

摘要

为研究尿液中白细胞产生白三烯B4（LTB4）的能力，我们评估了在不同条件下将人血多形核白细胞（PMNL）置于尿液中孵育后，钙离子载体A23187诱导的LTB4生成情况。将PMNL置于高pH或低pH及高渗透压的尿液中孵育后，LTB4的生成显著减少。长时间孵育也会抑制其生成。LTB4生成与PMNL活力之间存在显著相关性（r = 0.82，p < 0.01）。我们的结果表明，仅通过测定PMNL活力就有可能评估尿中PMNL的LTB4生成能力。此外，尿液酸化会阻碍LTB4的生成，这在体内可能是不利的。