Favorov M O, Jue D L, Hine T K, Fields H A
Hepatitis Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
Virology. 1994 Jan;198(1):390-3. doi: 10.1006/viro.1994.1048.
Using a set of 11 synthetic peptides containing regions of the polypeptide encoded by open reading frame 2 (ORF2) of the hepatitis E virus (HEV) genomic RNA, two immunodominant regions were found. One region is located at position 546-580 amino acids (aa). Another very strong immunodominant region was identified at position 394-470 aa. Five peptides spanning this region were found to have antigenic reactivity. One of these 5 peptides demonstrated specific reactivity with 81% of sera obtained from HEV-infected patients. To elucidate the antigenic structure of this region in fine detail, an additional set of 35 overlapped 20-mer peptides spanning the region 388-493 aa was synthesized. Two subregions with very strong antigenic reactivity, one located around position 420-440 aa and another around 450-460 aa, were identified. Thus, in addition to the strong epitope(s) in the C-terminal region of the ORF2 protein previously identified, at least two immunodominant regions were found at positions 394-470 and 546-580 aa.
使用一组包含戊型肝炎病毒（HEV）基因组RNA开放阅读框2（ORF2）编码的多肽区域的11种合成肽，发现了两个免疫显性区域。一个区域位于第546 - 580个氨基酸（aa）位置。另一个非常强的免疫显性区域在第394 - 470个氨基酸位置被鉴定出来。发现跨越该区域的5种肽具有抗原反应性。这5种肽中的一种与81%的戊型肝炎病毒感染患者血清表现出特异性反应。为了详细阐明该区域的抗原结构，又合成了一组35种重叠的20肽，其跨越第388 - 493个氨基酸区域。鉴定出两个具有非常强抗原反应性的亚区域，一个位于约第420 - 440个氨基酸位置，另一个位于约450 - 460个氨基酸位置。因此，除了先前鉴定的ORF2蛋白C端区域的强表位外，在第394 - 470和546 - 580个氨基酸位置还发现了至少两个免疫显性区域。
