Kotzé J P, Spies J H
S Afr Med J. 1976 Oct 9;50(43):1760-4.
The intravenous administration of heparin-released lipoprotein lipase (LPL) into the circulatory system of the baboon (Papio ursinus) is described. After a single heparin injection, a peak value of LPL activity appeared in the circulation with 5 minutes. At low doses of heparin (less than 100 units heparin/kg body mass), LPL disappeared from the circulation in an exponential fashion with a half-life of about 20 minutes. An increase in the heparin dose increased the amount of LPL released into the circulation. In baboons which were deficient in ascorbic acid, less LPL was released into the circulation after specific doses of heparin than in animals that were amply supplied with this vitamin (ascorbic acid 16 mg/kg body mass/day). The separation of plasma LOL, released by heparin, on Sephadex G-150, revealed several distinct molecular species of LPL in the eluant from the columns. In vitro studies indicated that ascorbic acid inhibited cardiac LPL strongly, whereas it had little effect on "post-heparin plasma" LPL. 2somolar concentrations of another reducing agent, mercapto-ethanol, slightly stimulated cardiac LPL in baboons.
本文描述了将肝素释放的脂蛋白脂肪酶（LPL）静脉注射到狒狒（山魈）循环系统中的情况。单次注射肝素后，5分钟内在循环系统中出现LPL活性峰值。在低剂量肝素（低于100单位肝素/千克体重）时，LPL以指数方式从循环系统中消失，半衰期约为20分钟。增加肝素剂量会增加释放到循环系统中的LPL量。在缺乏抗坏血酸的狒狒中，特定剂量肝素后释放到循环系统中的LPL比充分供应该维生素（抗坏血酸16毫克/千克体重/天）的动物少。通过葡聚糖凝胶G - 150对肝素释放的血浆LOL进行分离，在柱洗脱液中显示出几种不同分子形式的LPL。体外研究表明，抗坏血酸强烈抑制心脏LPL，而对“肝素后血浆”LPL影响很小。2毫摩尔浓度的另一种还原剂巯基乙醇对狒狒心脏LPL有轻微刺激作用。
