Zapletal O, Piskac A, Malá J, Drazan J
Vet Med (Praha). 1976;21(6):321-30.
The authors demonstrate the production of the B1 aflatoxin by means of a strain of the fungus Aspergillus flavus which was captured on cereal feed in spring 1975. Besides the production of the mycotoxin, this fungus strain showed a very quick and intensive growth and development, in comparison with the laboratory strains of Aspergillus flavus. The obtained and isolated aflatoxin B1 could be identified by chromatography and spectral photometry only when the fungus strain was cultivated on a suitable substrate where it showed the lowest producting of the distrubing fluorescent metabolism by-products. That finding led to the conclusion that in some cases it is necessary, besides the classically used procedure of the diagnosis of aflatoxicosis, to require also the isolation of the possibly present strain of Aspergillus flavus and--after its recultivation in pure cultures on suitable media--the determination of aflatoxin production connected with their physical and chemical identification.
作者通过1975年春季在谷物饲料上捕获的一株黄曲霉证明了B1黄曲霉毒素的产生。除了产生霉菌毒素外，与黄曲霉的实验室菌株相比，该真菌菌株显示出非常快速和密集的生长与发育。只有当该真菌菌株在合适的底物上培养时，所获得和分离的黄曲霉毒素B1才能通过色谱法和光谱光度法进行鉴定，在这种底物上它产生的干扰性荧光代谢副产物最少。这一发现得出的结论是，在某些情况下，除了经典的黄曲霉毒素中毒诊断程序外，还需要分离可能存在的黄曲霉菌株，并在合适的培养基上进行纯培养再培养后，测定与它们的物理和化学鉴定相关的黄曲霉毒素产量。
