使用铟-111标记的单克隆抗体MOC-31对裸鼠体内人小细胞肺癌异种移植瘤进行放射免疫检测。
Radioimmunodetection of human small cell lung carcinoma xenografts in the nude rat using 111in-labelled monoclonal antibody MOC-31.
作者信息
de Jonge M W, Kosterink J G, Bin Y Y, Bulte J W, Kengen R A, Piers D A, The T H, de Leij L
机构信息
Department of Clinical Immunology, University of Groningen, The Netherlands.
出版信息
Eur J Cancer. 1993;29A(13):1885-90. doi: 10.1016/0959-8049(93)90543-o.
The applicability of mouse monoclonal antibody MOC-31 for in vivo radioimmunodetection of human small cell lung cancer (SCLC) was investigated in a nude rat xenograft model. MOC-31 is reactive with a 38 kD pancarcinoma membrane antigen. [111In]DTPA-MOC-31 showed good in vivo immunolocalisation to xenografted SCLC cells, whereas antigen-related uptake was low in normal rat tissues and in a control antigen-negative, human-derived tumour. Non-antigen-related uptake in the liver could be blocked by pretreatment with irrelevant antibody. It is concluded that MOC-31 can be used for radioimmunodetection of SCLC in vivo and may be suitable as a targeting device in patients.
在裸鼠异种移植模型中研究了小鼠单克隆抗体MOC-31在体内对人小细胞肺癌（SCLC）进行放射免疫检测的适用性。MOC-31与一种38 kD的泛癌膜抗原有反应。[111铟]二乙三胺五乙酸-MOC-31对异种移植的SCLC细胞显示出良好的体内免疫定位，而在正常大鼠组织和对照抗原阴性的人源肿瘤中，与抗原相关的摄取较低。肝脏中与抗原无关的摄取可通过用无关抗体预处理来阻断。得出的结论是，MOC-31可用于体内SCLC的放射免疫检测，并且可能适合作为患者的靶向装置。
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