Ling Z D, Webb B T, Yeoh E, Matheson D S
Department of Paediatrics, University of British Columbia, Vancouver, Canada.
Cytokine. 1993 Jul;5(4):379-85. doi: 10.1016/1043-4666(93)90071-c.
Previous experiments demonstrated that aggregated immunoglobulin and the Fc fragment of human IgG can induce interleukin-6 (IL-6) secretion from peripheral blood monocytes. The data herein indicate that Fc-induced IL-6 is modulated by IL-1, IL-4 and interferon (IFN-gamma). When added with Fc fragments, IL-1 and IFN-gamma increased IL-6 production. IL-4 added with Fc fragment did not influence IL-6 production although IL-4 added with LPS was inhibitory to IL-6 production. However, when PBMC were pre-treated with IL-4, IL-4 downregulated Fc-induced IL-6 secretion. The inhibitory effect of IL-4 in the pre-treatment phase could be overcome with a high concentration of IFN-gamma added with the IL-4. Both IL-4 and IFN-gamma acted in a dose- and time-dependent manner. By dot blot analysis, IL-6 mRNA production appeared to be decreased in amount and duration by IL-4 whereas IFN-gamma increased the amount of IL-6 mRNA production. Hence, IL-4 and IFN-gamma appear to have opposing effects and may play a balancing role in the regulation of IL-6 production secondary to Fc exposure.
先前的实验表明，聚集的免疫球蛋白和人IgG的Fc片段可诱导外周血单核细胞分泌白细胞介素-6（IL-6）。本文数据表明，Fc诱导的IL-6受IL-1、IL-4和干扰素（IFN-γ）调节。与Fc片段一起添加时，IL-1和IFN-γ可增加IL-6的产生。与Fc片段一起添加的IL-4不影响IL-6的产生，尽管与LPS一起添加的IL-4对IL-6的产生有抑制作用。然而，当用IL-4预处理PBMC时，IL-4可下调Fc诱导的IL-6分泌。在预处理阶段，IL-4的抑制作用可通过与IL-4一起添加高浓度的IFN-γ来克服。IL-4和IFN-γ均呈剂量和时间依赖性作用。通过斑点印迹分析，IL-4似乎使IL-6 mRNA的产生量和持续时间减少，而IFN-γ增加了IL-6 mRNA的产生量。因此，IL-4和IFN-γ似乎具有相反的作用，并且可能在Fc暴露后IL-6产生的调节中起平衡作用。
