Cappadocia M, Heizmann P, Dumas C
Institut de Recherche en Biologie Végétale, Université de Montréal, Québec, Canada.
Plant Mol Biol. 1993 Dec;23(5):1079-85. doi: 10.1007/BF00021823.
In this study, the tissue printing technique has been used to rapidly localize in female tissues the presence of specific mRNA representing the products (or some of the products) of the self-incompatibility S-locus gene(s). The methodology, initially developed for Brassica oleracea (sporophytic self-incompatibility) has been successfully employed on Solanum chacoense (gametophytic self-incompatibility). In the Brassica system tissue printing has allowed rapid discrimination between S alleles belonging to class 1 (dominant types) vs. class 2 (recessive types), and thus parallels findings obtained by restriction analyses. In the Solanum system the level of the S-RNase messages was analysed by scanning laser densitometry, and it was found that the message levels of the allele S14 declined faster than those coming from S13 in mature flowers.
在本研究中，组织印迹技术已被用于在雌性组织中快速定位代表自交不亲和S位点基因产物（或部分产物）的特定mRNA的存在。该方法最初是为甘蓝（孢子体自交不亲和）开发的，现已成功应用于查科茄（配子体自交不亲和）。在甘蓝系统中，组织印迹能够快速区分属于第1类（显性类型）和第2类（隐性类型）的S等位基因，因此与通过限制性分析获得的结果相似。在茄属系统中，通过扫描激光密度测定法分析了S-RNase信息的水平，发现在成熟花中，等位基因S14的信息水平比来自S13的信息水平下降得更快。
