通过免疫金银染色以及在硝酸纤维素纸上进行组织印迹，对发育中的大豆种皮中伸展蛋白进行免疫细胞定位。
Immunocytolocalization of extensin in developing soybean seed coats by immunogold-silver staining and by tissue printing on nitrocellulose paper.
作者信息
Cassab G I, Varner J E
机构信息
Department of Biology, Washington University, St. Louis, MO 63130.
出版信息
J Cell Biol. 1987 Dec;105(6 Pt 1):2581-8. doi: 10.1083/jcb.105.6.2581.
Abstract
In soybean seed coats the accumulation of the hydroxyproline-rich glycoprotein extensin is regulated in a developmental and tissue-specific manner. The time course of appearance of extensin during seed development was studied by Western blot analysis and by immunogold-silver localization. Using these techniques extensin was first detected at 16-18 d after anthesis, increasing during development to high levels at 24 d after anthesis. Immunogold-silver localization of extensin in the seed coat showed marked deposition of the glycoprotein in the walls of palisade epidermal cells and hourglass cells. The immunolocalization of extensin in developing soybean seeds was also made by a new technique--tissue printing on nitrocellulose paper. It was found that extensin is primarily localized in the seed coat, hilum, and vascular elements of the seed.
摘要
在大豆种皮中，富含羟脯氨酸的糖蛋白伸展蛋白的积累受到发育和组织特异性方式的调控。通过蛋白质免疫印迹分析和免疫金银定位技术研究了伸展蛋白在种子发育过程中的出现时间进程。利用这些技术，在开花后16 - 18天首次检测到伸展蛋白，在发育过程中逐渐增加，在开花后24天达到高水平。种皮中伸展蛋白的免疫金银定位显示，该糖蛋白在栅栏状表皮细胞和沙漏状细胞的细胞壁中有明显沉积。伸展蛋白在发育中的大豆种子中的免疫定位也通过一种新技术——在硝酸纤维素纸上进行组织印迹来完成。结果发现，伸展蛋白主要定位于种子的种皮、种脐和维管组织中。
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本文引用的文献
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