Miura T, Muraoka S, Ogiso T
Department of Biochemistry, Hokkaido Institute of Pharmaceutical Sciences, Otaru, Japan.
Biochem Mol Biol Int. 1993 Sep;31(1):125-33.
When bovine serum albumin was exposed to the hydroxyl radical generating system of ascorbate-EDTA-Fe3+ or ascorbate-EDTA-Fe(3+)-H2O2, carbonyl formation occurred. Trolox strongly inhibited the oxidative modification in a dose-dependent manner. The fragmentation and enhancement of the proteolytic susceptibility of bovine serum albumin were induced by HO. only in the presence of O2, while trolox protected the protein against such oxidative modifications. In addition, inactivations of lactate dehydrogenase and creatine kinase induced by the hydroxyl radical were also blocked by trolox.
当牛血清白蛋白暴露于抗坏血酸 - 乙二胺四乙酸 - Fe3+ 或抗坏血酸 - 乙二胺四乙酸 - Fe(3+) - H2O2 的羟基自由基生成体系时，会发生羰基形成。生育三烯酚（Trolox）以剂量依赖的方式强烈抑制氧化修饰。仅在有 O2 存在时，羟基自由基（HO）诱导牛血清白蛋白的片段化并增强其对蛋白水解的敏感性，而生育三烯酚保护蛋白质免受此类氧化修饰。此外，生育三烯酚还能阻止羟基自由基诱导的乳酸脱氢酶和肌酸激酶的失活。
