人二倍体成纤维细胞的无血清条件培养基含有一种能刺激培养的牛主动脉内皮细胞产生前列环素的活性物质。
Serum-free conditioned medium of human diploid fibroblast cells contains an activity that stimulates prostacyclin production by cultured bovine aortic endothelial cells.
作者信息
Yamauchi T, Umeda F, Masakado M, Ono Y, Nawata H
机构信息
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
出版信息
Biochem Mol Biol Int. 1993 Sep;31(1):65-71.
Serum-free culture medium conditioned by human diploid fibroblast cells stimulated prostacyclin production by cultured bovine aortic endothelial cells. Gel filtration chromatography using Sephacryl S-100HR revealed at least three peaks of prostacyclin-stimulating activity. This factor was relatively heat-stable, acid-labile, trypsin-sensitive and bound to heparin Sepharose CL-6B. This factor was completely inhibited by indomethacin.
由人二倍体成纤维细胞条件化的无血清培养基刺激培养的牛主动脉内皮细胞产生前列环素。使用Sephacryl S - 100HR进行凝胶过滤色谱分析显示至少有三个前列环素刺激活性峰。该因子相对耐热、对酸不稳定、对胰蛋白酶敏感且与肝素琼脂糖CL - 6B结合。该因子被吲哚美辛完全抑制。
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