Haaheim L R
Acta Pathol Microbiol Scand B. 1976 Jun;84(3):159-61. doi: 10.1111/j.1699-0463.1976.tb01919.x.
The previously described method of assaying the antibody response to influenza virus by single-radial-immunodiffusion is based on the formation of opalescent zones when immune sera are allowed to diffuse into gels containing large amount of purified influenza virus. The zones are not thought to be the result of immune aggregates of virus particles and antibody since the virus is immobile in the gel and the average distance between two neighbouring particles is approximately 50 times the virus diameter. The present study investigates how different rabbit IgG-fragments react in this type of test. It was found that IgG, F(ab')2 and reduced (monovalent) IgG gave rise to distinct zones of opalescence. Fab', on the other hand, produced weak and hazy zones which could not be quantitated with any accuracy. We conclude that the most likely mechanism responsible for the zones is a light-scattering effect caused by antibodies attached to the viral surface and that the quality of the opalescence to some extent seems to be dependent on the Fc-fragment.
先前描述的通过单放射免疫扩散测定对流感病毒抗体反应的方法，是基于当免疫血清扩散到含有大量纯化流感病毒的凝胶中时形成乳光区。这些区域被认为不是病毒颗粒与抗体免疫聚集的结果，因为病毒在凝胶中是固定不动的，且相邻两个颗粒之间的平均距离约为病毒直径的50倍。本研究调查了不同的兔IgG片段在这类试验中的反应情况。结果发现，IgG、F(ab')2和还原型（单价）IgG产生了明显的乳光区。另一方面，Fab'产生的是微弱且模糊的区域，无法准确进行定量。我们得出结论，造成这些区域的最可能机制是附着在病毒表面的抗体引起的光散射效应，并且乳光的质量在一定程度上似乎取决于Fc片段。
