Papadimitriou J M, van Bruggen I
Department of Pathology, University of Western Australia, Nedlands.
Exp Mol Pathol. 1993 Aug;59(1):1-12. doi: 10.1006/exmp.1993.1022.
DNA fragmentation in murine monocytes and freshly harvested resident peritoneal macrophages (RPM) was assessed. Similar proportions of fragmented DNA were present in both cell types indicating that apoptosis is a significant occurrence in these cells and may provide a mechanism for controlling the numbers of mononuclear phagocytes in the tissues that house them. Culturing both of these cell types at 41 degrees C for 5 hr revealed a significant increase in the proportion of fragmented DNA only in cultures containing RPM. These latter preparations when cytophotometrically assessed also displayed reduced Feulgen DNA and cytoplasmic naphthol yellow S staining, again confirming the presence of apoptosis. It is suggested that the induction of apoptosis by mild hyperthermia may therefore be due to the relative absence of a temperature-sensitive inhibitor of apoptosis in a significant proportion of resident peritoneal macrophages. Lastly, electron microscope assessment indicated that the apoptotic macrophages were engulfed by their nonaffected neighbors. Programmed cell death in macrophage populations followed by phagocytosis of the apoptotic cells may therefore be a mechanism for controlling macrophage numbers in mammalian organisms.
对小鼠单核细胞和新鲜收获的驻留腹膜巨噬细胞（RPM）中的DNA片段化进行了评估。两种细胞类型中均存在相似比例的片段化DNA，这表明细胞凋亡在这些细胞中是一个显著现象，并且可能为控制容纳它们的组织中单核吞噬细胞的数量提供一种机制。将这两种细胞类型在41摄氏度下培养5小时后发现，只有在含有RPM的培养物中，片段化DNA的比例才显著增加。当对这些后期制备物进行细胞光度测定评估时，也显示出福尔根DNA和细胞质萘酚黄S染色减少，再次证实了细胞凋亡的存在。因此，有人提出，轻度热疗诱导细胞凋亡可能是由于相当一部分驻留腹膜巨噬细胞中相对缺乏温度敏感的细胞凋亡抑制剂。最后，电子显微镜评估表明，凋亡的巨噬细胞被未受影响的相邻细胞吞噬。因此，巨噬细胞群体中的程序性细胞死亡随后凋亡细胞被吞噬可能是哺乳动物机体中控制巨噬细胞数量的一种机制。
