Mondino B J, Kessler E, Gipson L L, Brown S I
Arch Ophthalmol. 1976 Dec;94(12):2149-52. doi: 10.1001/archopht.1976.03910040809015.
Zinc ions were shown to reversibly inhibit the activity of Pseudomonas protease. Zinc sulfate was more effective topically in preventing corneal perforations by this enzyme than either cysteine or edetate disodium (Na2 EDTA). The reversibility of the enzyme inactivation by zinc sulfate and its efficacy in preventing perforations were used to estimate the duration of the enzyme activity in the cornea. The protease was found to retain its activity in the rabbit cornea for up to two days after intrastromal injection. The extent of corneal destruction in rabbit corneas infected with live Pseudomonas cells was significantly greater in eyes treated with zinc sulfate than in controls, precluding its use in a clinical setting.
锌离子可可逆性抑制铜绿假单胞菌蛋白酶的活性。硫酸锌局部应用时，在预防该酶导致的角膜穿孔方面比半胱氨酸或乙二胺四乙酸二钠（Na2 EDTA）更有效。硫酸锌使酶失活的可逆性及其预防穿孔的功效被用于估计该酶在角膜中的活性持续时间。发现蛋白酶在基质内注射后在兔角膜中可保持活性长达两天。用硫酸锌处理的兔眼感染活铜绿假单胞菌细胞后角膜破坏程度比对照组显著更大，这排除了其在临床环境中的应用。
