一种用于研究人细小病毒B19在骨髓细胞培养物中复制情况的原位杂交技术。
An in situ hybridization technique for the study of B19 human parvovirus replication in bone marrow cell cultures.
作者信息
Vassias I, Perol S, Coulombel L, Thebault M C, Lagrange P H, Morinet F
机构信息
Laboratory of Microbiology, Saint-Louis Hospital, Paris, France.
出版信息
J Virol Methods. 1993 Oct;44(2-3):329-38. doi: 10.1016/0166-0934(93)90067-2.
PMID:8263124
Abstract
An in situ hybridization technique using digoxigenin labelling was developed to study B19 infection. By using appropriate DNA probes, transcription of structural and non-structural genes was detected in bone marrow cell cultures. Such a simple system is useful to the study of B19-cell interactions in non-permissive cell lines.
摘要
开发了一种使用地高辛配体标记的原位杂交技术来研究B19感染。通过使用合适的DNA探针，在骨髓细胞培养物中检测到了结构基因和非结构基因的转录。这样一个简单的系统对于研究非允许细胞系中B19与细胞的相互作用很有用。
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