Foto F, Saag K G, Scharosch L L, Howard E J, Naides S J
Department of Internal Medicine, University of Iowa College of Medicine, Iowa City.
J Infect Dis. 1993 Mar;167(3):744-8. doi: 10.1093/infdis/167.3.744.
To determine if B19 infection persists in patients with chronic B19 arthropathy, acute B19 infection in adults was documented by IgM serology. Bone marrow aspirates were obtained 24-42 months after acute infection in 4 patients who developed chronic joint symptoms. DNA from bone marrow aspirates was amplified by polymerase chain reaction by using B19-specific DNA sequences in the viral capsid gene and probing with B19-specific oligonucleotides in Southern analysis. B19-specific DNA sequences were detected in all 4 chronic arthropathy patients compared with 0 of 7 anti-B19 IgM-, IgG- and 0 of 6 anti-B19 IgM-, IgG+ normal bone marrow donors. Three patients with serologically proven acute B19 infection and acute but nonchronic joint symptoms had B19 DNA detected in bone marrow aspirates 2-18 months after infection. These findings suggest that B19 arthropathy is associated with persistence of either B19 virus or select B19 DNA sequences.
为确定B19感染是否在慢性B19关节炎患者中持续存在，通过IgM血清学方法记录了成人急性B19感染情况。在4例出现慢性关节症状的患者急性感染后24至42个月获取骨髓抽吸物。利用病毒衣壳基因中的B19特异性DNA序列通过聚合酶链反应扩增骨髓抽吸物中的DNA，并在Southern分析中用B19特异性寡核苷酸进行探针检测。与7例抗B19 IgM-、IgG-的正常骨髓供者中0例以及6例抗B19 IgM-、IgG+的正常骨髓供者中0例相比，在所有4例慢性关节炎患者中均检测到B19特异性DNA序列。3例血清学证实为急性B19感染且有急性而非慢性关节症状的患者在感染后2至18个月的骨髓抽吸物中检测到B19 DNA。这些发现提示，B19关节炎与B19病毒或特定B19 DNA序列的持续存在有关。
