Baitella-Eberle G, Groscurth P, Zilla P, Lachat M, Müller-Glauser W, Schneider J, Neudecker A, von Segesser L K, Dardel E, Turina M
Department of Cardiovascular Surgery, University Hospital Zurich, Switzerland.
J Vasc Surg. 1993 Dec;18(6):1019-28.
The purpose of this study was to investigate in vivo the long-term development, differentiation, and proliferation of the subendothelial tissue on Dacron prostheses seeded with microvascular cells (MVC).
Autologous MVC from omental adipose tissue were seeded on 4 mm Dacron prostheses and the prostheses interposed in the carotid arteries of mongrel dogs for 5, 13, and 26 weeks.
Light and electron microscopic evaluation of patent seeded prostheses demonstrated an almost complete monolayer of endothelial cells and well-organized subendothelial tissue, whereas patent control prostheses were mainly covered by red and white thrombi, which were partially replaced by organized tissue with increased implantation time. The measurements of the thickness of the luminal cell layer in seeded and control grafts showed no statistically significant increase between 5 and 26 weeks of implantation. The subendothelial tissue of seeded prostheses demonstrated a time-dependent maturation of highly synthesizing myofibroblasts embedded in a collagen matrix to cells with features of smooth muscle cells located in a collagen-elastin matrix. In control grafts examined after 26 weeks the spontaneous endothelialization was accompanied by a delayed or incomplete maturation of subendothelial tissue.
Our study indicates that MVC seeded onto Dacron prostheses are able to generate a vascular wall that does not continue to proliferate after prolonged implantation and that increasingly resembles the wall of a normal artery in cell differentiation and intercellular matrix.
本研究旨在对植入微血管细胞（MVC）的涤纶人工血管内皮下组织的长期发育、分化和增殖进行体内研究。
将来自网膜脂肪组织的自体MVC接种到4毫米的涤纶人工血管上，并将人工血管植入杂种犬的颈动脉中5周、13周和26周。
对通畅的接种人工血管进行光镜和电镜评估显示，几乎完全是单层内皮细胞且内皮下组织排列良好，而通畅的对照人工血管主要被红色和白色血栓覆盖，随着植入时间的增加，血栓部分被机化组织替代。对接种和对照移植物管腔细胞层厚度的测量显示，在植入5至26周之间没有统计学上的显著增加。接种人工血管的内皮下组织显示出一种时间依赖性成熟过程，即高度合成的肌成纤维细胞嵌入胶原基质中逐渐成熟为具有平滑肌细胞特征的细胞，这些细胞位于胶原-弹性蛋白基质中。在26周后检查的对照移植物中，自发内皮化伴随着内皮下组织延迟或不完全成熟。
我们的研究表明，接种到涤纶人工血管上的MVC能够形成一种血管壁，在长期植入后不会继续增殖，并且在细胞分化和细胞间基质方面越来越类似于正常动脉壁。
