Lassner F, Becker M, Fansa H, Schaller E, Berger A
Clinic for Plastic, Hand and Reconstructive Surgery, Medical School Hannover, Germany.
Microsurgery. 1993;14(7):457-61. doi: 10.1002/micr.1920140708.
Preservation of peripheral nerves may, in the near future, play an important role in reconstructive surgery, especially if recent advances in immunosuppressive therapy are taken into account. Therefore, it has to be investigated whether peripheral nerves can be stored for some time after harvesting without diminishing their regenerative potential. Previous experiments of our group could demonstrate only little benefit of organ storage solution (HTK) or normal saline (NaCl 0.9%) to peripheral nerves when kept at cold ischaemia of 4 degrees C for 32 and 72 hours. In this presentation, we are reporting the results of peripheral nerve storage in Dulbecco's Modified Eagle Medium which has been used for Schwann cell culture. In 30 adult Sprague-Dawley rats, a 2.5 cm segment of the right sciatic nerve was harvested and kept at 4 degrees C for 14, 32, 72, and 120 hours. It was then reimplanted into the donor animal; regeneration quality was assessed clinically, histologically and morphometrically after 6 weeks. Best regeneration results were obtained in the 32 and 72 hour groups; regeneration here was comparable to the normal controls. These results are explained with the positive effect of nerve predegeneration.
在不久的将来，保留周围神经可能在重建手术中发挥重要作用，尤其是考虑到免疫抑制治疗的最新进展。因此，必须研究周围神经在收获后是否可以储存一段时间而不降低其再生潜力。我们小组之前的实验表明，当在4摄氏度的冷缺血条件下保存32小时和72小时时，器官保存溶液（HTK）或生理盐水（0.9%氯化钠）对周围神经的益处很小。在本报告中，我们报告了在用于雪旺细胞培养的杜氏改良 Eagle 培养基中保存周围神经的结果。在30只成年Sprague-Dawley大鼠中，切取右侧坐骨神经2.5厘米的节段，在4摄氏度下保存14、32、72和120小时。然后将其重新植入供体动物体内；6周后通过临床、组织学和形态学方法评估再生质量。在32小时和72小时组中获得了最佳的再生结果；此处的再生情况与正常对照组相当。这些结果可以用神经预变性的积极作用来解释。
