人结肠癌细胞中胸苷酸合成酶核糖核蛋白复合物的鉴定
Identification of a thymidylate synthase ribonucleoprotein complex in human colon cancer cells.
作者信息
Chu E, Voeller D M, Jones K L, Takechi T, Maley G F, Maley F, Segal S, Allegra C J
机构信息
NCI-Navy Medical Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892.
出版信息
Mol Cell Biol. 1994 Jan;14(1):207-13. doi: 10.1128/mcb.14.1.207-213.1994.
Abstract
Translation of thymidylate synthase (TS) mRNA is controlled by its own protein product, TS, in an autoregulatory manner. Direct binding of TS protein to two different cis-acting elements on the TS mRNA is associated with this translational regulation. In this study, an immunoprecipitation-reverse transcription-PCR technique was used to identify a TS ribonucleoprotein (RNP) complex in cultured human colon cancer cells. Using antibodies specific for TS protein, we show that TS is complexed in vivo with its own TS RNA. Furthermore, evidence demonstrating a direct interaction between the mRNA of the nuclear oncogene c-myc and TS protein is presented.
摘要
胸苷酸合成酶（TS）mRNA的翻译由其自身的蛋白质产物TS以一种自我调节的方式进行控制。TS蛋白与TS mRNA上两个不同的顺式作用元件的直接结合与这种翻译调控相关。在本研究中，采用免疫沉淀-逆转录-PCR技术在培养的人结肠癌细胞中鉴定出一种TS核糖核蛋白（RNP）复合物。使用针对TS蛋白的特异性抗体，我们证明TS在体内与其自身的TS RNA形成复合物。此外，还提供了证明核癌基因c-myc的mRNA与TS蛋白之间直接相互作用的证据。
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