Binger M H, Hug D, Weber G, Schildknecht E, Hümbelin M, Pasamontes L
Vitamin Research, VRD/F, F. Hoffmann-La Roche Ltd., Basel, Switzerland.
Mol Biochem Parasitol. 1993 Oct;61(2):179-87. doi: 10.1016/0166-6851(93)90064-5.
A rabbit serum raised against Eimeria tenella merozoites was used to screen a lambda gt11 cDNA library made from merozoite mRNA of E. tenella. The insert of the phage clone lambda Mz 5-7 revealed an open reading frame consisting of 945 nucleotides, encoding a 33-kDa protein. This size is consistent with the size of a protein translated in vitro from merozoite mRNA and immunoprecipitated with monospecific anti-Mzp 5-7 antibodies. A smaller protein of 24 kDa, located on the surface of the parasite, also reacted with the monospecific antiserum and is the potential processed form of the Mzp 5-7. Furthermore, a recombinant vaccinia virus expressing the Mzp 5-7 antigen was constructed and used to immunize chickens.
用针对柔嫩艾美耳球虫裂殖子制备的兔血清筛选由柔嫩艾美耳球虫裂殖子mRNA构建的λgt11 cDNA文库。噬菌体克隆λMz 5-7的插入片段显示一个由945个核苷酸组成的开放阅读框，编码一种33 kDa的蛋白质。这个大小与从裂殖子mRNA体外翻译并用单特异性抗Mzp 5-7抗体免疫沉淀的蛋白质大小一致。一种位于寄生虫表面的24 kDa较小蛋白质也与单特异性抗血清发生反应，是Mzp 5-7的潜在加工形式。此外，构建了表达Mzp 5-7抗原的重组痘苗病毒并用其免疫鸡。
