Harper S J, Walsh F S, Doherty P
Department of Experimental Pathology, UMDS, Guys Hospital, London, UK.
Neurosci Lett. 1993 Sep 3;159(1-2):202-6. doi: 10.1016/0304-3940(93)90834-8.
Embryonic chick spinal neurons have been cultured over sections of human foetal muscle to determine which cell adhesion molecules present in embryonic muscle are important in promoting neurite outgrowth. Using blocking antibodies against the major cell adhesion molecules, neural cell adhesion molecule (NCAM), N-cadherin and the beta 1 subunit of the integrins, neurite outgrowth was significantly blocked only by anti-integrin antibodies. In addition other agents that block neurite outgrowth stimulated by NCAM, N-cadherin and L1, such as the calcium channel antagonists verapamil and omega-conotoxin and pertussis toxin which inactivates G-proteins also had no effect. This suggests that in this culture system integrins are able to promote neurite outgrowth whereas NCAM and N-cadherin are not.
将鸡胚脊髓神经元培养在人胎儿肌肉切片上，以确定胚胎肌肉中存在的哪些细胞粘附分子对促进神经突生长很重要。使用针对主要细胞粘附分子神经细胞粘附分子（NCAM）、N-钙粘蛋白和整合素β1亚基的阻断抗体，只有抗整合素抗体能显著阻断神经突生长。此外，其他阻断由NCAM、N-钙粘蛋白和L1刺激的神经突生长的试剂，如钙通道拮抗剂维拉帕米和ω-芋螺毒素以及使G蛋白失活的百日咳毒素，也没有效果。这表明在该培养系统中，整合素能够促进神经突生长，而NCAM和N-钙粘蛋白则不能。
