[Synergism in the stimulating effect of transforming growth factor beta and insulin on the substrate-independent proliferation of the CHO-719 cell line].

Cells of line CHO-719 from the Chinese hamster ovary have no epidermal growth factor receptors. It was detected that these cells were significantly stimulated to proliferate in a semisolid culture medium containing 0.33% agar by transforming growth factor beta (TGF-beta) in combination with insulin. This effect was even more pronounced when the cells of line CHO-719, adapted to the growth in the presence of low concentration (3%) of fetal bovine serum, were used. TGF-beta and insulin utilized separately exerted no influence on the cellular growth.