将蛋白质电印迹到羧甲基纤维素膜上进行测序。
Electroblotting proteins onto carboxymethylcellulose membranes for sequencing.
作者信息
Alimi E, Martinage A, Sautière P, Chevaillier P
机构信息
Laboratoire de Biologie Cellulaire, Université Paris-Val de Marne, Créteil, France.
出版信息
Biotechniques. 1993 Nov;15(5):912-7.
PMID:8267986
Abstract
Proteins and peptides separated by polyacrylamide gel electrophoresis either in acetic acid-urea or in sodium dodecyl sulfate (SDS)-containing gels were electroblotted onto carboxymethylcellulose membrane and eluted from the membrane by dilute acid. Polypeptides thus recovered with a high yield can be used to get structural or biological data.
摘要
通过聚丙烯酰胺凝胶电泳在乙酸 - 尿素或含十二烷基硫酸钠（SDS）的凝胶中分离得到的蛋白质和肽，被电印迹到羧甲基纤维素膜上，并用稀酸从膜上洗脱下来。以高产量回收的多肽可用于获取结构或生物学数据。
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