Ash S R, Carr D J, Blake D E, Rainier J B, Demetriou A A, Rozga J
Hemocleanse, Inc., Purdue Research Park, W. Lafayette, Indiana 47906.
ASAIO J. 1993 Jul-Sep;39(3):M675-80.
An experimental model of hepatic failure in the dog has been developed in which the liver is devascularized in two stages. Under general anesthesia, a portacaval shunt is created, ligatures placed around the hepatic and gastroduodenal arteries, and the dog recovered. Two days later under general anesthesia, the ligatures are pulled, converting hepatic insufficiency to hepatic failure. Five control animals developed hypotension, severe lactic acidosis, hypoglycemia, and increasing liver enzyme levels during 6 hrs of follow-up. The BioLogic-DT system includes a cellulosic plate dialyzer with a suspension of powdered charcoal and cation exchangers as dialysate. Five animals were treated with the BioLogic-DT for 6 hrs after creation of hepatic failure. These animals were more stable physiologically, developed less lactic acidosis and less enzyme elevation, and maintained high normal blood glucose levels. The results help explain the clinical improvement demonstrated in patients with hepatic failure treated by the BioLogic-DT, and confirm that many of the toxins of hepatic failure are dialyzable and bound by simple sorbents such as charcoal and cation exchangers.
已建立一种犬肝衰竭实验模型，在此模型中肝脏分两个阶段进行去血管化处理。在全身麻醉下，建立门腔分流，在肝动脉和胃十二指肠动脉周围放置结扎线，然后让犬恢复。两天后在全身麻醉下，拉动结扎线，将肝功能不全转变为肝衰竭。五只对照动物在6小时的随访期间出现低血压、严重乳酸酸中毒、低血糖以及肝酶水平升高。BioLogic-DT系统包括一个纤维素平板透析器，其透析液为粉末状活性炭和阳离子交换剂的悬浮液。五只动物在肝衰竭形成后用BioLogic-DT治疗6小时。这些动物生理上更稳定，乳酸酸中毒程度较轻，酶升高较少，并且维持较高的正常血糖水平。这些结果有助于解释接受BioLogic-DT治疗的肝衰竭患者所表现出的临床改善情况，并证实肝衰竭的许多毒素是可透析的，并且可被活性炭和阳离子交换剂等简单吸附剂结合。
